Microscopy

2-photon FLIM-FRET

• Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K. Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb;9(2):283-91. Epub 2006 Jan 22.

  • Gratton E, Breusegem S, Sutin J, Ruan Q, Barry N. Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods. J Biomed Opt 2003 Jul; 8(3):381-90.

• Yasuda R, Minchinsky EA, Scheuss V, Pologruto TA, Ortner TG, Sabatini BL, Svoboda K. Imaging calcium concentration dynamics in small neural compartments. Science STKE 2004.

2-photon methods

• • Forli … Fellin et al. Two-Photon Bidirectional Control and Imaging of Neuronal Excitability with High Spatial Resolution In Vivo. Cell Reports. 22, 3087-3098

Structured Illumination Microscopy

• Strategic and practical guidelines for successful structured illumination microscopy. Demmerle et al. Nature Protocols 2017 doi:10.103.8/nprot.2017.019