Please keep all areas of the histology hall clean. If a solution is running low, please alert Vikko (firstname.lastname@example.org).
Please log all brains on the follow form: https://docs.google.com/spreadsheets/d/1Hnk-VXwZ7fK3y3SbjfIfBfXe9gqagCBkhs6_0Kff9Qc/edit?usp=sharing
This will help us declutter the fridge space and keep track of brains that are sitting around for a longer time.
Please label all brains with the following information:
Ferret Number/Shave Pattern
Date Transferred to ___ (PFA-> 10% Sucrose -> 30% Sucrose).
A comprehensive list of histological reagents can be found here: https://docs.google.com/spreadsheets/d/1xWuZ0Rg9_VfMdmPUnUmchtaUjjuy2VBV7KWbI2zfDE4/edit#gid=0
Inhibitory Interneuron Project (Julie Miller's Old Stuff) :
i: imaged; R: ROIs taken; cc: cell count done
[iteration 8]: December 2012
[iteration 7]: December 2012 i
[iteration 6]: December 2012 i
[iteration 5]: December 2012 i
[iteration 4]: November - December 2012 i, R
[iteration 3]: November 2012 i
[iteration 2]: August 2012 i
[iteration 1]: July 2012 i, R, cc
Notes by Month:
> May 2012
> Feb 2012
Notes by Antigen:
Division of Science Summer Undergrad Poster Session 2012 [.pdf]
0910-12: [DS Laminar Development]
Nissl Staining Summer/Fall 2016
Brain 1144 lesion #1: 1316 #2: 256
Brain 1148 lesion #1: 1118 #2: 314
Brain 1149 lesion #1: 1036 #2: 274
Brain 1160 lesion #1: 1241 #2: 283
SMI-32 & NeuN Staining Summer/Fall 2016
Keyence BX-Z 710 is a Fluorescence Microscope that supports brightfield, phase contrast, oblique illumination, and fluorescence observation all within a single compact unit.
The Keyence scope has 3 channels (DAPI, GFP, and Texas Red). If you want to use it to image your brain slices, ensure that your fluorophores are within those working ranges. (Image taken from ThermoFisher.com - Fluorescence SpectraView
Zeiss LSM 880:
LSM880 2+1Ch: confocal microscope with 7 laser lines (405/458/488/514/561/594/633nm) and 3 detectors of which 1 a GaAsP detector (for green-yellow detection, where GaAsP has highest sensitivity, however, you can also direct other spectra to this detector.