Golgi-Cox staining allows for visualization of whole neurons, including complete dendritic arbors and dendritic spines. Golgi can be used on a variety of neural tissues, ranging from rodent to postmortem human tissue. Typically, cells appear darkly stained against an amber or yellow background. One advantage of Golgi staining is that it is resistant to many factors that would cause other staining methods to fail. The staining procedure is, while long in duration, mostly hands-off and is suitable for a beginner. The primary drawbacks are the hazardous reagents required for Golgi-Cox solution.
For the most up-to-date protocol for using Golgi-Cox staining, see the file below.