PERFUSION SOLUTION RECIPE USING 10% FORMALIN
***Make this under the hood***
In a 500 mL flask, mix:
*make this solution the day you plan to perfuse, and dispose of any excess in the waste container under the hood. Do not store.
If 10% Formalin is not available:
PERFUSION SOLUTION RECIPE USING PARAFORM
In a 500 mL flask:
Heat 220 mL dH20 to 60 degrees C
Add 20 g paraform (weigh out under hood; wear mask)
Stir for 10 minutes
Add NaOH while stirring (~1 pellet). Solution so turn mostly clear.
Add 30 mL dH2O
Add 250 mL 0.2M phosphate buffer