Ferret perfusion

PERFUSION SOLUTION RECIPE USING 10% FORMALIN


***Make this under the hood***


In a 500 mL flask, mix:

  • 50 mL 10% Formalin solution (found under hood)’

  • 250 mL 0.2M phosphate buffer

  • 200 mL dH20


*make this solution the day you plan to perfuse, and dispose of any excess in the waste container under the hood. Do not store.


If 10% Formalin is not available:


PERFUSION SOLUTION RECIPE USING PARAFORM


In a 500 mL flask:

  • Heat 220 mL dH20 to 60 degrees C

  • Add 20 g paraform (weigh out under hood; wear mask)

  • Stir for 10 minutes

  • Add NaOH while stirring (~1 pellet). Solution so turn mostly clear.

  • Add 30 mL dH2O

  • Add 250 mL 0.2M phosphate buffer