Expired protocols

IACUC Protocols

These are expired or retired IACUC protocols. See the current protocols here.

2019
19010 (replacing 13009, 13011, 14003, and 16003) Van Hooser development, function & organization of cortical circuits (APPROVED until 2022-04-05)
- - Title: Understanding the development, function, and organization of mammalian cortical circuits
  - Required CITI modules: Question 4 - Select learner group for FERRETS (to select ferrets, pick learner group 6, then select the ferret module from the non-bolded pop-up list). Should be 1) Aseptic surgery, 2) Working with the IACUC, and 3) Working with Ferrets in Research Settings.
  - Personnel: David Bressler, Michela Grimes, Lauren Hayashi, Amanda Huang, Jonathan Joasil, Dan Lehman, Andrea Stacy, Steve Van Hooser, Shen Wang

- - Protocol documents:
    - Original Approved Protocol
    - Modification 1: Generic Pentobarbital-Phenytoin for euthanasia (dropping specific names Beuthanasia-D and Nembutal) [approval] [updated integrated protocol with changes] [updated integrated protocol, clean]
    - Modification 2: personnel
    - Modification 3: adding parturition and dark-rearing [approved modification]
    - Modification 4: Adding face scanning [approved modification]
    - Annual update 1: Approved through April 5, 2022
    - Modification 5: Prostaglandin and Oxytocin dosage changes [approved modification] [updated integrated protocol with changes] [updated integrated protocol]
    - Modification 7: refractive error correction [approved modification] [updated integrated protocol with changes] [updated integrated protocol]
    - Modification 8: catheterization of bladder, microchipping, photography [approved modification] [updated integrated protocol with changes] [updated integrated protocol]
    - Modification 9: food removal prior to surgery [approved modification] [updated integrated protocol with changes] [updated integrated protocol]
    - Modification 10: Clarifying dark rearing care, updating water deprivation procedures, clarifying humane endpoints for animals with chronic implants, adding 2 new rigs for 2P use [approved modification] [updated integrated protocol with changes] [updated integrated protocol]
    - Modification 11: Standard Vaccination of Dark Reared Animals [approved modification] [updated integrated protocol with changes] [updates integrated protocol]

2018
18006 (collaborative with Maya Shelly) (approved until 2021-07-14)
- - Title: Understanding the role of genes in the development of brain circuits

- On CITI: - Select learner group for MICE and RATS
  - Personnel: Stephen Van Hooser
- Protocol documents:
  - - Original Approved protocol
    - Modification 1: Adding Bassine 419 as short-term housing (approved 2019-03-14)
  - [Annual update #1, July 30, 2019 - July 14, 2021]
  - [Annual update #2, July 13, 2020 - July 14, 2021]

2017
- 17004 (replacing 14006) Paradis - Van Hooser mouse protocol (approved until 2020-05-31)
  - - Title: Understanding the roles of genes and experience in the development of brain circuits

- - Required CITI modules and SOPs:
  - On CITI: Question 4 - Select learner group for MICE
  - On CITI: No Biosafety training required.
    - Van Hooser lab Urethane SOP
      - Van Hooser lab Chlorprothixene SOP
    - Personnel: Stephen Van Hooser, Suzanne Paradis, Daniel Acker, Chelsea Groves Kuhnle, Anna Moore, Sarah Richards, Andrea Stacy, Vedakumar Tatavarty, Jada George, Karina Meythaler, Nia Lyn
  - Logs: Click here (email Steve for access)
  - Protocol documents:
    - - Original Approved protocol
      - Modification 1: Atipamezole [approved modification] [updated integrated protocol with changes] [updated integrated protocol, clean]
      - Modification 2: Whisker trimming [approved motification]
    - [Annual update #1 May 23 2018 - May 31 2019]
    - [Annual update #2 - until May 31 2020]
      - Modification 4: Awake recordings (approved Jan 6, 2020)

2016
- 16003 (replacing 13009, 13011, & 14003) Van Hooser development, function & organization of cortical circuits (APPROVED until 2019-04-12)
  - - Title: Understanding the development, function, and organization of mammalian cortical circuits
    - Required CITI modules: Question 4 - Select learner group for FERRETS (to select ferrets, pick learner group 6, then select the ferret module from the non-bolded pop-up list)
    - Personnel: Tudor Dragoi, Michela Grimes, Lauren Hayashi, Amanda Huang, Jonathan Joasil, Andrew Lipnick, Arani Roy, Nathan Schneider, Susannah Slepian, Andrea Stacy, Steve Van Hooser, Shen Wang, Derek Wise

- - Protocol documents:
    - Latest version, all modifications included (after modification 1)
    - Original Approved Protocol
    - [Annual Update #1 April 13, 2017 - April 12, 2019]
    - Modification 1: Slice procedures and Atipamezole [approved modification] [updated integrated protocol with changes] [updated integrated protocol, clean]
    - Modification 2: Chronic recordings [approved modification] [updated integrated protocol with changes] [updated integrated protocol, clean]
    - (Mod 3 was a personnel addition)
    - (Mod 4 was administrative adding of CRCNS)
    - Modification 5: option of Lidocaine analgesia [approved modification] [updated integrated protocol with changes] [updated integrated protocol, clean]

2015
- 15001 (formerly 1011-12) Van Hooser mouse/rat/ferret slice protocol (APPROVED UNTIL 2017-12-13)

- - On CITI: Question 4 - Select learner groups for MICE, RATS, and FERRETS (to select ferrets, pick learner group 6, then select the ferret module from the non-bolded pop-up list)
  - On CITI: No Biosafety training required.
  - Personnel: Jason Osik, Steve Van Hooser
  - Approved protocol (PDF) - [Annual Update #1 2015 - 2016][Annual Update #2 2016-2017]

2014
- 14006 Paradis / Van Hooser mouse protocol (APPROVED UNTIL 2017-06-03)

- - Required CITI modules and SOPs:
  - On CITI: Question 4 - Select learner group for MICE
  - On CITI: No Biosafety training required.
    - Van Hooser lab Urethane SOP
  - Personnel: Anna Moore, Sarah Richards, Arani Roy, Sue Paradis, Steve Van Hooser, Andrea Stacey
  - Logs: Click here (email Steve for access)
    - Approved Protocol [Annual renewal approval 1] [Annual renewal approval 2 until 2017-06-03]
  - Modification #1 for extra surgery for Aim 7 (mini-microscope)
  - Modification #2 for intrinsic signal imaging
    - Modification #3 for use of Rig 1 and use of anti-inflammatories
  - Modification #4 for use of yohimbine and isoflurane for all survival procedures
    - Modification #5 for use of chlorprothixene and neonatal virus injections
  - Modification #6 for use of SRGAP2 KO mice & extracellular recordings
  - Modification #7 for use of additional experimental space
  - Modification #8 for use of Diazepam
  - Modification #9 for use of retrograde dyes & mice lines
  - Modification #10 L4 Neurons

- 14003 [replaced by 16003] (formerly 1011-03) Fiser / Van Hooser awake intrinsic electrophysiology (APPROVED until 2016-10-06)

- - Required CITI modules
  - On CITI: Question 4 - Select learner group for FERRETS (to select ferrets, pick learner group 6, then select the ferret module from the non-bolded pop-up list)
  - On CITI: No Biosafety training required.
  - Personnel: Jozsef Fiser, Steve Van Hooser, Marjena Popovic, Arani Roy, Nora Anderson
  - Approved Protocol - [2013-2014 annual update] [2014-2015 annual update] [2015-2016 annual update #2]
  - Approved Protocol - 2012-2013 Annual update
  - 14003 Modification #1 - Awake training (approved 2014-05-22)
  - 14003 Modification #2 - Dark rearing (approved 2014-07-09)

2013
- 13009 [replaced by 16003] Van Hooser Tree shrew visual cortex physiology / imaging (APPROVED until 2016-04-27)

- - Required CITI modules
  - On CITI: Question 4 - Select learner group for RATS
  - On CITI: No Biosafety training required.
  - Personnel: Steve Van Hooser, Elizabeth Johnson, Heywood Petry
  - Approved Protocol, 2nd Annual update
  - Amendment 1: receive from MaxPlanck Florida Institute, tree shrews might be housed for more than a week
  - Amendment 2: change in pancuronium dose

- 13011 Van Hooser FUSION protocol [replaced by 16003] (replaces 0910-12 AND 1011-02 imaging and virus injection) (APPROVED until 2016-05-16)

- - Required CITI modules:
  - On CITI: Question 4 - Select learner group for FERRETS (to select ferrets, pick learner group 6, then select the ferret module from the non-bolded pop-up list)
  - On CITI: Question 5 - Select the option for non-IBC-member researchers (right now, that's Group 2).
  - Personnel: Steve Van Hooser, Shen Wang, Arani Roy, Jason Osik, Nora Anderson, Neil Ritter, Benyamin Meschede-Krasa
  - Approved Protocol
  - Annual Update 1
  - Annual Update 2
  - Amendment 1
  - Amendment 2
  - Amendment 3 / Modification 3 - allowing animals to be housed for only 5 days before surgery
  - Amendment 4 : allowing use of adult ferrets

2012
- 12012 Van Hooser (formerly Fiser) Probabilistic visual perceptíon and learning in the brain (CLOSED 2/2014)

- - Required CITI modules
    - Investigators, Staff and Students, Basic Course
    - Working with Rats in Research Settings, Basic Course (because there's no ferret course)
    - Aseptic Surgery
    - Reducing Pain and Distress in Laboratory Mice and Rats, Basic Course
    - Personnel: Steve Van Hooser, Marjena Popovic
  - Approved Protocol (2012-2013 annual update)
    - Administrative amendment 1:
    - Administrative amendment 2:
  - Administrative amendment 3: Taking over from Fiser

2011
- 1011-12 Van Hooser mouse/rat/ferret slice protocol (APPROVED until 2013-06-21)

- - Required CITI modules:
    - Investigators, Staff and Students, Basic Course
    - Working with Rats in Research Settings, Basic Course
    - Working with Mice in Research Settings, Basic Course
    - Working with Ferrets in Research Settings, Basic Course
    - Reducing Pain and Distress in Laboratory Mice and Rats, Basic Course
    - Personnel: Jason Osik, Steve Van Hooser
  - 1011-12 modification #2
    - 1011-12 Van Hooser mouse/rat slice Annual Updat3 7/23/13
  - 1011-12 Van Hooser mouse/rat slice protocol Annual Update #1
  - 2011-06-08 submission.doc

- 1011-02 Van Hooser ferret virus IACUC protocol (APPROVED until 2013-05-25) associated with IBC 11009

- - Required CITI modules:
    - Investigators, Staff and Students, Basic Course
    - Animal Biosafety, Stage 1
    - Reducing Pain and Distress in Laboratory Mice and Rats, Basic Course
    - Working with Rats in Research Settings, Basic Course (because there's no ferret course)
    - Aseptic Surgery
    - OSHA Personal Protective Equipment Training, Stage 1
    - Training for Investigators, Staff and Students Handling Biohazards., Stage 1
  - Exact Title (8/8/2011): The Functional Role of Experience in the Development of Visual Cortical Circuits
    - Personnel: Jason Osik, Neil Ritter, Heather Bernstein, Jennifer Wolff, Steve Van Hooser, Jared Clemens, Arani Roy, Johanna Ledley
  - 1011-02 CURRENT YEAR, UPDATE 1 Van Hooser Ferret Virus protocol.pdf
  - 1011-02 FIRST YEAR, OLD VERSION Van Hooser Ferret Virus protocol.pdf
  - Amendment 1: injectable-only anesthesia
  - Amendment 2: isoflurane earlier without xylazine
  - Amendment 3: Perfusions without physiology allowed, post-op survival of 6 weeks
  - Amendment 4: The use of Amniograft to reduce scarring in brain tissues
  - Amendment 5: Use of tramadol and extended survival period (up to 10 weeks)

- 11009 Van Hooser AAV / Lentivirus in Ferrets (APPROVED 2018-06-26 UNTIL 2019-06-25)
  - - Approved IBC Protocol 11009 [2018-2019 update] [2019-2020 update]
- 11009 Van Hooser Optogenetics in Ferret (APPROVED until 2013-09-12) associated with IACUC 1011-02

- - Van Hooser IACUC Virus ChR2 ferret protocol original submission 2010-10-29.doc [Revised 2011-01-01]

- 11009 Van Hooser Optogenetics in Ferret (APPROVED until 2013-09-12) associated with IBC 11009

- 1011-06 Lau / Paradis / Van Hooser xenopus imaging protocol (APPROVED until 2014-03-17)

- - Required CITI modules:
    - - Investigators, Staff and Students, Basic Course

- - - 1011-06 Lau / Paradis / Van Hooser protocol document 2011-03-05
    - Personnel: Amy Ghiretti, Anna Moore, Jared Clemens, Arani Roy, Sue Paradis, Nelson Lau, Steve Van Hooser

- - Current version approved March 18, 2013

- 12020 Turrigiano / Van Hooser rat / mouse 2-photon calcium imaging protocol (APPROVED until 2013-05-3) April/2012: 1011-09 is now combined with 0809-46 and 0809-35 to make 12020. Protocol will be updated below when approved.
  - - Required CITI modules and SOPs:
      - Investigators, Staff and Students, Basic Course
    - Reducing Pain and Distress in Laboratory Mice and Rats, Basic Course
      - Working with Mice in Research Settings, Basic Course
      - Working with Rats in Research Settings, Basic Course

- - 12020 Turrigiano protocol PDF
    - Personnel: Gina Turrigiano, Steve Van Hooser, Sandrine Lefort, Arani Roy, Melanie Gainey, Mary Lambo, Anna Chavez, Kelly Flavahan

- 1011-05 Paradis / Van Hooser mouse histology and mouse imaging (APPROVED until 2014-03-13)
  - - Required CITI modules and SOPs:
      - Investigators, Staff and Students, Basic Course
      - Working with Mice in Research Settings, Basic Course
    - Reducing Pain and Distress in Laboratory Mice and Rats, Basic Course
      - Van Hooser lab Urethane SOP
    - Update 1011-05 Paradis / Van Hooser 2013-03-14.pdf
    - Update 1011-05 Paradis / Van Hooser 2012-02-16.pdf
    - Amendment #3 2012-12-13.pdf
    - Amendment #4 2013-08-12.pdf
    - Personnel: Anna Moore, Sandrine Lefort, Amy Ghiretti, Arani Roy, Sue Paradis, Steve Van Hooser
    - Logs: Click here (email Steve for access)
2010
- 0910-12 Van Hooser non-survival ferret electrophysiology/imaging protocol (APPROVED until 2013-06-04)
  - - Required CITI modules:
      - Investigators, Staff and Students, Basic Course
      - Working with Rats in Research Settings, Basic Course (because there's no ferret course)
    - Reducing Pain and Distress in Laboratory Mice and Rats, Basic Course
    - 0912-12 Van Hooser 2012-06-05 (current version)
    - Previous submissions/amendments:
    - 0910-12 Van Hooser 2010-06-23 - Protocol.pdf
    - 0910-12 Van Hooser Personnel Amendment 1 2010-10-19.pdf
    - 0910-12 Van Hooser Amendment 1 2010-10-28.pdf
    - 0910-12 Van Hooser Amendment 2 2010-11-04.pdf
    - 0910-12 Van Hooser Amendment 3 2011-02-07.pdf
    - 0910-12 Van Hooser Amendment 4 2011-04-19.pdf
    - 0910-12 Van Hooser Amendment 5 2012-01-12.pdf
      - You know how in The Prisoner (the McGoohan version, please), he's talking with number 2, who keeps insisting "You are number Six," but in the end, he's not, and you catch yourself thinking "Wait, who was number 6, then?" This is kind of like that. There is no number 6.
      - 0910-12 Van Hooser Amendment 7 2012-02-17.pdf (adding protein staining on tissue from euthanized animals for any reason)
    - Personnel: Jason Osik, Jared Clemens, Arani Roy, Heather Bernstein, Jenn Wolff, Steve Van Hooser, Marjena Popovic, Neil Ritter, Victor Zhivich, Ameya Apte, Dan Rubin (Dan until June 23 2011), Johanna Ledley

IBC Protocols

11008 Van Hooser AAV in Rats (APPROVED until 2013-02-22)
- Required CITI modules:
- On CITI: Question 4 - Select learner group the animals you're working with (will depend on the protocol number, above).
- On CITI: Question 5 - Select the option for non-IBC-member researchers (right now, that's Group 2).
11008 Van Hooser AAV in Rats (APPROVED until 2013-02-22) associated with IBC 10008
- Approved IBC Protocol 10008
- IBCattachA_AAV_in_Rats_12142010_SVH.doc
- IBCattachB_AAV_in_Rats_12142010_SVH.doc
- IBCcover_AAV_in_Rats_12142010_SVH.pdf
- Personnel: Jenn Wolff